• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The invention relates to a composition for the decontamination and inoculation of surfaces, the composition comprising an antimicrobial agent and a mixture of stabilized bacteria. The composition can be used to inoculate the surfaces for the purpose of purifying the surfaces of unwanted, harmful or pathogenic microorganisms.
    公开号:BE1026987B1
    申请号:E20205047
    申请日:2020-01-23
    公开日:2020-08-25
    发明作者:Corrie Gielen；Robin Temmerman
    申请人:Chrisal；
    IPC主号:
    专利说明:

    Field of the Invention The present invention relates to compositions for the decontamination and inoculation of surfaces. The invention also relates to a corresponding method for its use. Introduction It is known that in some cases traditional cleaning products and methods are not sufficient or efficient enough. For example, in hospitals, supplies such as furniture, floors, and patient beds and mattresses must be cleaned regularly and thoroughly to reduce the number of pathogenic microorganisms that can affect the health of recovering patients. In addition, for obvious reasons, shared facilities, such as industrial kitchens, and components thereof, as well as industrial facilities and components, such as filters, pumps, and ducts, also require permanent monitoring and reduction of such pathogenic populations. It is known that probiotics can be used to combat these harmful bacteria. WO 2006/125283 teaches that colonizing surfaces with non-pathogenic spore-forming bacteria via cleaning products has a beneficial effect on reducing the number of pathogenic microorganisms on such surfaces. This reduces the risk of infections and has a beneficial effect on general health.
    However, given the growing resistance of some strains of bacteria to certain cleaning products, it is extremely important to minimize the numbers of such pathogens. There is therefore a need for products and methods that can minimize unwanted, harmful or pathogenic microorganisms on a surface. Summary of the Invention According to one aspect of the present invention, there is provided a composition for the decontamination and inoculation of surfaces, the composition comprising an antimicrobial agent and probiotic component, the probiotic component being a mixture of stabilized bacteria.
    It is an advantage of the present invention that the composition of the invention can be used to reduce populations of harmful or pathogenic microorganisms. Furthermore, it has been found that the combination of an antimicrobial agent, such as a disinfectant, and a probiotic component has a stimulating effect on the reduction of these pathogenic population concentrations with respect to decontamination processes based on a product containing only an antimicrobial agent or a probiotic component. It was further advantageously found that repeated cycles of decontamination using the composition, in an accelerated manner, result in a permanently low concentration of the pathogen population on the surface, as compared to surface decontamination with compositions containing an antimicrobial agent only or a probiotic. component. In one embodiment of the composition of the present invention, the probiotic component comprises at least one of Bacillus spores, lactic acid bacteria, soil bacteria, water bacteria, human microbiome bacteria or plant bacteria.
    In one embodiment of the composition of the present invention, the probiotic component is a mixture of Bacillus spores.
    It is an advantage of this embodiment that spores of species of the genus Bacillus are easy to manipulate and can be used in a product with a shelf life of at least three years.
    In one embodiment of the composition of the present invention, the Bacillus spore mixture is present at a concentration of 1 x 10 ° to 1 x 10 1 cfu per gram of composition.
    It is an advantage of this embodiment that the concentration range allows both the decontamination of surfaces using a detergent or cleaning product comprising the composition and the decontamination of surfaces by water dosing, with products for the latter usually having an initially higher cfu concentration. per gram of composition due to the fact that these products are often diluted in use.
    In one embodiment of the composition of the present invention, the composition further comprises at least one prebiotic.
    It is an advantage of this embodiment that the use of at least one prebiotic results in a further reduction in the concentration of the pathogen population. It has further been found that adding at least one prebiotic to a probiotic component increases the rate of germination - and thus of activity - of the latter.
    In one embodiment of the composition of the present invention, the at least one prebiotic comprises at least one of a fructooligosaccharide, galactooligosaccharide, and / or inulin.
    In one embodiment of the composition of the present invention, the at least one prebiotic is present at a concentration of 100 mg to 100 g per kg of composition.
    In one embodiment of the composition of the present invention, the antimicrobial agent is selected from the group consisting of alcohols, aldehydes, oxidizing agents, peroxyacids, phenols, quaternary ammonium compounds, chlorine compounds, iodine compounds, acid and bases, metals and terpenes, or mixtures thereof.
    In one embodiment of the composition of the present invention, the antimicrobial agent is present at a concentration of 0.001% to 90% by weight, based on the total weight of the composition.
    In one embodiment of the composition of the present invention, the probiotic component and the prebiotic agent are at least partially microencapsulated.
    It is an advantage of this embodiment that the use of microcapsules further allows germination to be spread over time, ensuring a constant presence and sustained activity of bacteria on the treated surface.
    In one embodiment of the composition of the present invention, the microcapsule comprises a friable shell and is further filled with a non-aqueous solution.
    In one embodiment of the composition of the present invention, the composition is formulated as a detergent, cleaning product, liquid, emulsion, cream, ointment, lotion, gel, oil, solution, aerosol spray, powder or semi-solid formulation. It is an advantage of this embodiment that the composition can be used as part of or formulated as the above products, thereby increasing the range of application of the composition. Advantageously, the products usually have a shelf life of at least three years.
    In one embodiment of the composition of the present invention, the surface is wood, paper or textile; the surface is an abiotic material, such as stone, metal, plastic, glass, carbon or ceramic; or is the surface a composite material. It is an advantage of this embodiment that the composition can be applied to a variety of surfaces, which are usually abiotic. However, with the exception of human or animal skin, this composition can also be applied to some biotic materials.
    According to an aspect of the present invention, there is provided a method of treating surfaces with the composition for the simultaneous decontamination and inoculation of surfaces, the method comprising the steps of cleaning, spraying, ultrasonic atomizing, brushing or water dosing the surfaces with the composition. According to one aspect of the present invention, there is provided a cleaning product comprising the composition.
    Brief Description of the Figures Figure 1 shows the effect of cleaning products on the count of Staphylococcus aureus populations.
    Detailed Description of Embodiments The invention described herein relates to a composition for the decontamination and inoculation of surfaces, the composition comprising an antimicrobial agent and a probiotic component.
    A specific use of the composition according to embodiments of the invention is a composition for the decontamination and inoculation of surfaces of non-living things.
    It is noted that the term "non-living thing" can also refer to that which used to be part of a living being, such as wood, rubber, paper and the like.
    However, the term "surface" here does not refer to the surface of living things, thereby excluding human skin, animal skin or fur and the like.
    More specifically, such a surface can be a surface made of or comprising wood, paper, leather, textile; the surface can be a surface made of or comprising an abiotic material, such as stone, metal (and alloys thereof), plastic and other polymeric materials, glass, ceramics, bricks and the like.
    Furthermore, the surface may be a composite material surface, the term "composite material" herein referring to material made of two or more constituent materials.
    The surface may further be a mixture of the above materials.
    In embodiments, the surface may be a surface that is exposed to air, or that is partially or completely immersed in water or another liquid. For the purpose of the invention, the term "surface decontamination" herein refers to working on a surface for the purpose of purifying that surface, meaning the reduction of unwanted, harmful or pathogenic microorganisms on the surface, such as bacteria, fungi, yeasts, viruses and the like. For the purpose of the invention, the term "inoculation of surfaces" refers herein to the act of introducing microorganisms, more particularly a probiotic component, onto the surface.
    For these purposes, the surface decontamination and inoculation may comprise the act of cleaning the surface with the composition, or by applying the composition to the surface by spraying, ultrasonic atomization, brushing, or water dosing.
    For the purpose of the invention, the term "water dosing" refers herein to the purification of surfaces in installations and / or surfaces of components thereof by adding the composition to a liquid, such as a cooling liquid, a rinsing liquid or a circulating liquid, wherein the liquid is in contact with the surface to be treated. Surfaces of pools and ponds, as well as surfaces of industrial installations, such as the inner surface of pipes, pumps and filters, can be subject to biofilm growth and organic waste accumulation. The composition can thus be added to the liquid in contact with the surface.
    Where appropriate, the invention will be described with reference to such uses, without intending to limit the scope of the invention thereto. For the purpose of the present invention, the term "antimicrobial agent" refers to an agent that destroys or inhibits the growth of microorganisms, especially pathogenic microorganisms. Disinfectants are considered a class of antimicrobial agents.
    In accordance with embodiments of the invention, the antimicrobial agent is selected from the group consisting of alcohols, aldehydes, oxidizing agents, peroxyacids, phenols, quaternary ammonium compounds, chlorine compounds, iodine compounds, acid and bases, metals and terpenes, or mixtures thereof.
    According to embodiments of the invention, the antimicrobial agent is present at a concentration of at least 0.001% by weight, preferably at least 0.01% by weight, more preferably at least 0.1% by weight, even more preferably at least 1% by weight and most preferably at least 5% by weight based on the total weight of the composition. It will further be appreciated that the antimicrobial agent is present at a concentration of at most 90% by weight, preferably at most 80% by weight, more preferably at most 70% by weight, even more preferably at most 60% by weight, more preferably at most preferably at most 50% by weight, even more preferably at most 40% by weight and most preferably at most 30% by weight, based on the total weight of the composition.
    For the purpose of the present invention, the term "synbiotic" refers to a combination of a probiotic and a prebiotic.
    For the purpose of the present invention, the terms "prebiotic" or "at least one prebiotic" refer to at least one of a short chain sugar (usually 3-9 monosaccharide units).
    In specific embodiments, the prebiotic contains one or more of the group consisting of galactooligosaccharides, fructooligosaccharides, and inulin.
    In preferred embodiments, the prebiotic is inulin.
    According to preferred embodiments of the invention, the at least one prebiotic has a proportion by mass that is at least 0.5 mg, preferably at least 1 mg, more preferably at least 10 mg, even more preferably at least 100 mg and most preferably a minimum of 1 g, per kg of the composition. It will further be understood that the at least one prebiotic has a proportion by mass that is at most 300 g, preferably at most 200 g, more preferably at most 100 g, and most preferably at most 80 g, per kg of the composition.
    For the purpose of the present invention, the term "probiotic" or "probiotic component" refers herein to a mixture of stabilized species of the domain Bacteria.
    According to embodiments of the invention, the probiotic component comprises at least one of the following: spores of at least one non-pathogenic species of the genus Bacillus, lactic acid bacteria, soil bacteria, aquatic bacteria, human microbiome bacteria or plant bacteria; such as, but not limited to Lactobacillus, Lactococcus, Bifidobacterium, Azospirillum, Enterobacter, Klebsiella, Pseudomonas, Sphingomonas, Nitrospira, Pedomicrobium, Mycobacterium, Nocardia, Desulfovibrio, and Sulphuricurvum. In preferred embodiments of the invention, the probiotic component comprises a mixture of spores from different non-pathogenic Bacillus species (hereinafter referred to as Bacillus spp.). In preferred embodiments of the invention, the probiotic component comprises at least one of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens.
    Preferably, the probiotic component comprises a mixture of spores of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens. For a mixture comprising spores of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens, each of the four Bacillus species preferably has a number of spores containing at least 10%, preferably at least 15%, and more preferably at least 20%, with respect to the total number of spores of the four Bacillus spp. in the mixture. It is further understood that each of the four Bacillus species has a spore count that is at most 70%, preferably at most 60%, more preferably at most 60%, even more preferably at most 50%, preferably at most 40 %, more preferably at most 35%, and most preferably at most 30%, with respect to the total number of spores of the four Bacillus spp. in the mixture. Preferably, traces of the four species are present in substantially or exactly equal amounts in the mixture. In accordance with specific embodiments of the invention, the mixture further comprises Bacillus polymyxa. In accordance with specific embodiments of the invention, the blend does not include Bacillus coagulans. In preferred embodiments, the total amount of spores in the probiotic component, which comprises a mixture of spores of different non-pathogenic Bacillus species, ranges from 1x10 ° to 1x10 ° C per gram of the composition. Such amount is expressed as cfu (colony forming units). It is clear that this value refers to the total number of cfu of all Bacillus spp together.
    In certain embodiments, the probiotic component is a mixture consisting of spores of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens.
    The transformation of spores into vegetative cells results from absorption of moisture from the surrounding surface or from the biofilm to which the preparation is applied [Knudsen et al. (2015) J. Bacteriol. 19; 198 (1): 168-77]. This moisture absorption decreases the absolute moisture content of the surface or the biofilm, thereby inhibiting the availability of moisture for other microorganisms. The vegetative Bacillus cells produce a variety of extracellular enzymes on surfaces and in biofilm that reduce the amount of organic nutrients on the surface and in biofilm [Priest (1977) Bacteriol. Rev. 41 (3): 711-53]. This reduction exerts an inhibitory effect on the availability of food to other microorganisms through nutrient depletion and the general concept of competitive exclusion [Ragione et al. (2003) Vet. Microbiol. 94 (3): 245-56].
    Treating a surface with a decontamination composition containing a probiotic component can affect the concentration of unwanted and potentially harmful microorganisms. In a particularly interesting embodiment, such an effect can be further optimized by selecting a mixture of the above four Bacillus spp., I.e. Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens. Because these Bacillus spp. have a different germination speed, therefore also have a different concentration distribution over time, a constant presence of the probiotic component on the surface to be treated is ensured. This choice will result in an even more pronounced fluid depletion, as well as an optimized diversity of extracellular enzymes.
    It is an advantage of the present invention that the composition described herein can be used to reduce populations of harmful or pathogenic microorganisms.
    More specifically, the combination of an antimicrobial agent, such as a disinfectant, and a probiotic component has the effect that these concentrations of pathogenic populations can be lowered to a low level, as compared to compositions containing only a microbial agent or a probiotic component. contain. Repeating surface decontamination at fixed times with a composition according to the invention advantageously resulted in a permanent concentration of the pathogen population, the concentration being low compared to surface decontamination with compositions containing only a disinfectant or a probiotic component.
    Furthermore, it was unexpectedly found that the addition of a prebiotic component to such a composition enhanced the effect of the composition even further, by accelerating the decontaminating effect as well as further reducing the concentration of pathogens on the treated surface.
    The surface decontamination and inoculation composition can be used to purify a surface of organic soil, which may be in the form of a biofilm matrix, as well as to reduce the number of other microorganisms such as bacteria, viruses, yeasts and fungi. The addition of at least one prebiotic to the probiotic component has been found to further enhance the purifying effect of the composition, with the prebiotic component appearing to support and enhance the effect of the probiotics on biofilm removal and appearing to other microorganisms to lower.
    In accordance with embodiments of the invention, at least one of the probiotic component, the antimicrobial agent and the at least one prebiotic agent may be at least partially encapsulated in microcapsules.
    For the purpose of the invention, microcapsules are particles containing at least one of the probiotic component, the antimicrobial agent and the at least one prebiotic agent.
    In preferred embodiments, the microcapsule includes the probiotic component and the at least one prebiotic. Such capsules are described in US 20120076864. Advantageously, these components can be encapsulated in such microcapsules to provide the composition with a longer stability / shelf life. Furthermore, after a "free" first portion of the probiotic component and, if present, the at least one prebiotic agent of the composition, which portion was not microencapsulated, has already exerted the effect, the microcapsules in a delayed release of provide a second portion of the probiotic and the at least one prebiotic agent on the surface or biofilm. As a result, a persistent activity of the composition up to 6 weeks can be obtained, provided that sufficient friction is present.
    If the preparation is thereby applied to a surface that is subject to friction (such as human or animal activity (walking, touching) or mechanical activity (interaction with machines or vehicles and the like)), the presence of the microcapsules is an established way of ensure that the preparation exerts a long-lasting action.
    As a result, it is advantageous to include such microcapsules in the composition for the decontamination and inoculation of surfaces where frequent surface treatment is not possible.
    Typically, the microcapsules are composed of a brittle shell containing a liquid containing the spores. The shell breaks open by friction and releases the contents of the capsule. The microcapsules usually have a diameter of 1 to 300 µm.
    According to embodiments of the invention, the casing is composed of a polymer layer, such as a polymer of gelatin, polyurethane, polyolefins, polyamides, polyesters, polysaccharides, silicone resins, epoxy resins, chitosan and aminoplastic resins such as a melamine-formaldehyde resin.
    In accordance with embodiments of the invention, the probiotic component and the at least one prebiotic agent may be present together or separately in a microcapsule. However, microcapsules can also be provided that contain individual strains of bacteria.
    In accordance with embodiments of the invention, the liquid in the capsules is usually non-aqueous and more specifically immiscible with water (e.g., an organic oil, a silicone oil, a fluorocarbon, or mixtures thereof). In certain embodiments, the liquid may contain the antimicrobial agent.
    The weight ratio of the casing to the liquid contained therein is usually 1: 500 to 1: 5000. The outermost layer of this shell may contain functional reactive groups for chemical bonding.
    In accordance with embodiments of the invention, up to 10% by weight, 20% by weight, 30% by weight, 50% by weight or even 75% by weight of the probiotic component and / or the at least one prebiotic may be encapsulated in the microcapsules. Microcapsules can be dispersed in a liquid or viscous composition.
    According to embodiments of the invention, the composition is processed into cleaning products (detergents), or products for spraying, for (ultrasonic) atomization, for brushing or for water dosing. Such products are usually in liquid form.
    Furthermore, depending on the type of treatment and the type of surface in question, the composition can be used as a component in or formulated as: a detergent, a cleaning product, a liquid, an emulsion, a cream, an ointment, a lotion, a gel, an oil, a solution, a trigger spray, an aerosol spray, a powder or a semi-solid formulation. Advantageously, the composition can be incorporated into a product for nebulization, preferably ultrasonic nebulization, and combined with a nebulizing device. An ultrasonic atomization device refers herein to a device that uses high frequency sound vibrations to produce an extra fine water mist which is then expelled to add moisture to the chamber. Such an application has been found to allow surface treatment of surfaces that cannot be achieved with conventional cleaning techniques or that cannot be treated by means of water dosing.
    Compositions for decontamination and inoculation of surfaces by (ultrasonic) atomization and methods therefor can be of particular interest for the treatment of HVAC installation surfaces, in particular the surface of the inner sides of channels. It was found that the installation of an ultrasonic nebulizing device in the duct of an air-conditioning installation, in which the device nebulizing a surface decontamination composition according to the invention, had a beneficial effect on the presence of harmful microorganisms.
    In preferred embodiments, water droplets have an average diameter ranging from 0.5 to 10 µm.
    It was found that under these conditions the composition could be maximally distributed in an available space.
    The invention further relates to a method for treating surfaces with the composition for the simultaneous decontamination and inoculation of surfaces as described above. Part of the composition may be microencapsulated. The method further comprises the steps of cleaning (in the sense of freeing dirt from contamination), spraying, ultrasonic atomizing, brushing or water dosing the surfaces with the composition according to methods known in the art.
    The invention further relates to a cleaning product comprising the composition as described herein.
    While the invention has been described above with reference to specific embodiments, this is done to illustrate and not to limit the invention, the scope of which is defined by the appended claims.
    Those skilled in the art will readily understand that other combinations of features than those described herein are possible without departing from the scope of the claimed invention.
    Experimental Results The invention will now be described in more detail with reference to the following examples, the purpose of which is only illustrative and is not intended to limit the scope of the invention.
    Galacto-Oligosaccharides: Ergomax GOS Fructo-Oligosaccharides: Supersmart Fructo Inulin: Beneo Orafti Inulin cfu count was performed using ISO 20391-1: 2018. Example 1 (E1) - Comparative Examples 1-2 (CE1-CE3) - Cleaning product A universal cleaning product was defined, with one of the following compositions, as summarized in Table 1: Table 1 - Cleaning product compositions
    Le AA Od Ginie bop OE B Por 0 TE aa mes ea OO RE Ge OEE Gomi EEE where the components of the cleaning product are expressed by weight and where the Probiotic Mixture is an aqueous mixture of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Bacillus amyloliquefaciens . Each component of the probiotic mixture is present at a concentration of 2 x 10 ° cfu per gram of the total composition, which means that each component of the mixture has a number of spores that is 25% with respect to the total number of spores. Example 2 (E2) - Purification of a surface Universal cleaning products according to the composition E1 (a synbiotic disinfectant), CE1 (which forms a dilute detergent), CE2 (a concentrated disinfectant) and CE3 (a probiotic composition) were applied daily on a floor in a confined experimental room under controlled conditions for a total duration of 10 days.
    Microbiological samples were taken from the surface of the floor to determine the presence of the hospital bacterium Staphylococcus aureus. Such samples were taken at 1, 6, 24, 48, 72, 120 and 240 hours. From 24 hours onwards, samples were taken just prior to product application to measure the effect of the previous 24 hours of application.
    The results in Figure 1 show the following:
    - The application of the diluted cleaning agent ("control" cleaning product according to composition CE1) does not appear to affect the level of S. aureus CFUs. - The application of the concentrated disinfectant ("disinfectant" - cleaning product according to composition CE2) results in a rapid decrease of S. aureus colony-forming units (CFU). However, this effect is only short-lived because the S. aureus count returns to the initial control level after a few hours. Application of the probiotic cleaning product ("probiotic" cleaning product according to composition CE3) results in a decrease of S. aureus CFUs after 24 hours to reach a minimum level (i.e. 430 cfu / m2) after 240 hours. - Application of the synbiotic disinfectant ("synbiotic disinfectant" - cleaning product according to composition E4) leads to an immediate and steep decrease of S. aureus CFUs within the hour, this decrease being maintained for at least the next 10 days, resulting in a final level of 220 cfu / m after 240 hours.
    权利要求:
    Claims (15)
    [1]
    A composition for the decontamination and inoculation of surfaces, the composition comprising an antimicrobial agent and a probiotic component, the probiotic component being a mixture of stabilized bacteria.
    [2]
    The composition of claim 1, wherein the probiotic component comprises at least one of Bacillus spores, lactic acid bacteria, soil bacteria, water bacteria, human microbiome bacteria or plant bacteria.
    [3]
    Composition according to claim 1 or 2, wherein the probiotic component is a mixture of Bacillus spores.
    [4]
    The composition of claim 3, wherein the Bacillus spore mixture is present at a concentration of 1 x 10 5 to 1 x 10 1 cfu per gram of composition,
    [5]
    A composition according to any preceding claim, wherein the composition further comprises at least one prebiotic.
    [6]
    The composition of claim 5, wherein the at least one prebiotic comprises at least one of a fructooligosaccharide, a galactooligosaccharide and / or inulin.
    [7]
    A composition according to claim 5 or claim 6, wherein the at least one prebiotic is present at a concentration of 100mg to 100g per kg of composition.
    [8]
    A composition according to any of the preceding claims, wherein the antimicrobial agent is selected from the group consisting of alcohols, aldehydes, oxidizing agents, peroxyacids, phenols, quaternary ammonium compounds, chlorine compounds, iodine compounds, acid and bases, metals and terpenes, or mixtures thereof. .
    [9]
    A composition according to any of the preceding claims, wherein the antimicrobial agent is present at a concentration of 0.001% to 90% by weight, based on the total weight of the composition.
    [10]
    The composition of any of claims 5 to 9, wherein the probiotic component and the at least one prebiotic agent are at least partially microencapsulated.
    [11]
    The composition of claim 10, wherein the microcapsule comprises a friable shell and is additionally filled with a non-aqueous solution.
    [12]
    A composition according to any of the preceding claims, wherein the composition is formulated as a detergent, cleaning product, liquid, emulsion, cream, ointment, lotion, gel, oil, solution, aerosol spray, powder or semi-solid formulation.
    [13]
    Composition according to any one of the preceding claims, wherein the surface is wood, paper or textile; wherein the surface is an abiotic material, such as stone, metal, plastic, glass, carbon or ceramic; or wherein the surface is a composite material.
    [14]
    A method for treating surfaces with the composition for the simultaneous decontamination and inoculation of surfaces according to any one of claims 1 to 13, the method comprising the steps of cleaning, spraying, ultrasonic nebulizing, brushing or water dosing the surfaces with the composition.
    [15]
    A cleaning product comprising the composition of any of claims 1 to 14.
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    同族专利:
    公开号 | 公开日
    EP3685669A1|2020-07-29|
    BE1026987A1|2020-08-17|
    引用文献:
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    法律状态:
    2020-10-12| FG| Patent granted|Effective date: 20200825 |
    优先权:
    申请号 | 申请日 | 专利标题
    EP19153405.6A|EP3685669A1|2019-01-23|2019-01-23|Composition for the decontamination and inoculation of surfaces|
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